Table 1.
Chemically synthesized CoA-thioesters. Products were confirmed by HPLC-MS. Yield was determined with HPLC-UV at 260 nm.
| Acyl-CoA | Yield (%) | Used Method 1 |
|---|---|---|
| Acetyl-CoA 1 | 81 | anhydride |
| Propionyl-CoA 2 | 86 | anhydride |
| Butyryl-CoA 3 | 87 | anhydride |
| Hexanoyl-CoA 4 | 76 | CDI |
| Heptanoyl-CoA 5 | 74 | CDI |
| Octanoyl-CoA 6 | 68 | CDI |
| Lauryl-CoA 7 | 67 | CDI |
| Acrylyl-CoA 8 | 17 | ECF |
| 3,3-Dimethylacrylyl-CoA 9 | 39 | ECF |
| Crotonyl-CoA 10 | 80 | anhydride |
| Octenoyl-CoA 11 | 57 | ECF |
| Sorbityl-CoA 12 | 61 | ECF |
| Cinnamoyl-CoA 13 | 75 | ECF |
| Isobutyryl-CoA 14 | 68 | CDI |
| 2-Methylbutyryl-CoA 15 | 78 | CDI |
| 3-Hydroxypropionyl-CoA 16 | 66 | CDI |
| 3-(R)-Hydroxybutyryl-CoA 17 | 54 | CDI |
| 3-(S)-Hydroxybutyryl-CoA 18 | 57 | CDI |
| 6-Oxoheptanoyl-CoA 19 | 56 | CDI |
| Succinyl-CoA 20 | 86 | anhydride |
| Methylsuccinyl-CoA 21 | 40 | CDI |
1 If multiple synthesis methods were successful only the one with the highest yield is listed (see also Table S1).