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Anticoagulant activity of thrombin-binding aptamers. Apt P demonstrates much better anticoagulant activity than Apt A. Anticoagulant activity improves when the aptamers are linked via a 20-base ssDNA linker, with the B-P pair showing much better anticoagulation than the B-A pair. The concentration of construct in each case is 1 µM. Clotting time is expressed as mean ± SEM.
