Figure 6.

Schematic of regulation cycles. Aptamer turns off the coagulation cascade (red circles), while antidote reverses inhibition and turns on coagulation (green circles). Coagulation can be turned off again by addition of retriever I strand which binds to the overhang in the antidote and displaces the aptamer, which regains its folded structure and inhibits thrombin. Similarly, retriever II strand can displace antidote using the overhang on retriever I. The now free antidote, once again, binds to and deactivates aptamer, turning coagulation back on.