Table 1.
Intra- and inter-day precision (% RSD) and accuracy (% Bias) of the HPLC-PDA method for determination of maleic acid in rat blood and kidney dialysates (6 days, 6 replicates per day).
| Nominal Concentration (µg/mL) | Intra-Day | Inter-Day | ||||
|---|---|---|---|---|---|---|
| Observed Concentration (µg/mL) | Precision (% RSD) | Accuracy (% Bias) | Observed Concentration (µg/mL) | Precision (% RSD) | Accuracy (% Bias) | |
| Plasma | ||||||
| 0.5 | 0.48 ± 0.01 | 1.13 | −4.21 | 0.52 ± 0.04 | 7.75 | 3.06 |
| 1 | 0.99 ± 0.01 | 1.15 | −0.83 | 1.00 ± 0.08 | 7.70 | 0.07 |
| 5 | 5.01 ± 0.02 | 0.41 | 0.11 | 5.06 ± 0.08 | 1.57 | 1.22 |
| 10 | 10.1 ± 0.04 | 0.38 | 0.92 | 9.88 ± 0.26 | 2.59 | −1.18 |
| 25 | 25.0 ± 0.01 | 0.05 | −0.15 | 25.0 ± 0.10 | 0.39 | 0.11 |
| Kidney | ||||||
| 0.5 | 0.51 ± 0.03 | 6.37 | 1.47 | 0.51 ± 0.03 | 5.57 | 1.90 |
| 1 | 1.02 ± 0.04 | 3.84 | 1.83 | 0.99 ± 0.07 | 6.89 | −0.69 |
| 5 | 4.96 ± 0.04 | 0.81 | −0.73 | 5.03 ± 0.22 | 4.43 | 0.50 |
| 10 | 10.1 ± 0.15 | 1.47 | 0.71 | 9.94 ± 0.27 | 2.69 | −0.62 |
| 25 | 25.0 ± 0.04 | 0.16 | −0.04 | 25.0 ± 0.09 | 0.36 | 0.00 |
Data expressed as mean ± SD.