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Effect of hLYS on H. pylori membrane permeabilization. H. pylori was incubated with (A) PBS (a control) or (B) 50 μM PI and 0.3 mg/mL hLYS for 90 min at room temperature under microaerophilic conditions. The cells were observed using fluorescence confocal microscopy. (A) No fluorescence was observed, indicating that PI could not enter the cells; (B) A few red fluorescence dots could be observed, demonstrating that PI could enter cells and bind to DNA.
