Table 1.
The chromatographic parameters of chiral separation of cetirizine enantiomers along with paraben M under various conditions on CHIRAL-HSA column.
| Mobile Phase | Retention Factor k | Enantioselectivity Factor α | Resolution Factor Rs | |||||
|---|---|---|---|---|---|---|---|---|
| Organic Solvent (A) | PB a mM pH 7 (B) | A:B (v/v) | Flow-Rate (mL·min−1) | Temp. (°C) | PM b | (S)-Cetirizine | ||
| 2-propanol | 10 | 14:86 | 0.9 | 25 | 2.72 | 2.65 | 1.10 | 0.80 |
| 2-propanol | 10 | 10:90 | 0.9 | 25 | 3.27 | 3.23 | 1.43 | 1.82 |
| 0.7 | 25 | 4.85 | 4.75 | 1.32 | 1.62 | |||
| 0.5 | 25 | 5.78 | 5.85 | 1.20 | 1.40 | |||
| 0.9 | 18 | 5.10 | 4.98 | 1.30 | 1.45 | |||
| 2-propanol | 10 | 6:94 | 0.9 | 25 | 5.75 | 5.82 | 1.21 | 1.54 |
| 2-propanol | 20 | 6:94 | 0.9 | 25 | 5.65 | 5.61 | 1.25 | 1.59 |
| acetonitrile | 10 | 10:90 | 0.9 | 25 | 3.05 | 3.10 | 1.15 | 0.65 |
| acetonitrile | 10 | 6:94 | 0.9 | 25 | 3.82 | 3.90 | 1.26 | 1.12 |
| acetonitrile | 20 | 6:94 | 0.9 | 25 | 3.78 | 3.77 | 1.25 | 1.10 |
| acetonitrile | 10 | 5:94 | 25 | 5.80 | 5.90 | 1.38 | 1.65 | |
| 0.9 | 18 | 7.45 | 7.54 | 1.28 | 1.38 | |||
| acetonitrile | 10 | 4:96 | 0.9 | 25 | 6.88 | 6.75 | 1.32 | 1.45 |
| acetonitrile | 20 | 4:96 | 0.9 | 25 | 6.70 | 6.62 | 1.38 | 1.48 |
a PB—phosphate buffer; b PM—paraben M.