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. 2015 Dec 24;21(1):22. doi: 10.3390/molecules21010022

Figure 6.

Figure 6

dPGS-ICC binding to human serum. (A) Fluorescence lifetime curves of 0.1 µM dPGS-ICC alone and with 10%, 30%, 50%, and 70% human serum in DPBS at 20 °C; (B) Fluorescence decay curves for titration of dPGS-ICC with human serum at different concentrations between 0.001% and 10% serum; (C,D) Fractional saturation as a function of human serum concentration both linear (C) and with a logarithmic x-axis (D). The half maximum binding concentration was determined to be 0.3% human serum (using a Hill-coefficient of n = 1) for a single component fit and 0.2% and 5% for a double component fit.