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. 2016 Jul 29;21(8):993. doi: 10.3390/molecules21080993

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of L. edodes extract on the expression of various cytokines in the ear. The ears were excised on day 28 and total RNA was isolated. Quantitative real-time polymerase chain reaction was performed as described in the Materials and Methods. The relative fold change in mRNA for IL-4 (A); IL-13 (B); IL-17 (C); IL-22 (D); IL-31 (E); and TNFα (F) are shown. Data are presented as the mean ± SD of triplicate determinations. * Significantly different from the value of the DFE/DNCB-treated mice at p < 0.05. DFE, Dermatophagoides farinae extract; DNCB, 2,4-dinitrochlorobenzene; AD, atopic dermatitis induced by DFE and DNCB treatment.