Figure 9.

HPLC chromatographs of Punica granatum peel ethanolic extract hydrogel-based formulation (PGHF), showing retention times for punicalin (4.203 min) and punicalagin (5.987 min). Chromatography was run on a Shimadzu LC-20AT HPLC with SPD-M20A PDA detection. Separations were carried out on a Dr. Maisch GmbH C18 column (5 μm, 250 mm × 4.6 mm), 25 °C, with a mobile phase of 0.1% (v/v) aqueous solution of phosphoric acid and acetonitrile (80:20). The mobile phase flow rate was 1 mL/min with 10 min run time. The standards were detected using a wavelength of 254 nm. Sample injection volume was 10 μL.