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. 2016 May 30;21(6):683. doi: 10.3390/molecules21060683

Figure 4.

Figure 4

Figure 4

Protection effects of AR against H/R-induced H9c2 cardiomyocytes apoptosis. Pretreatment with AR (1, 10, 100 μM) for 24 h prior to H/R, morphology and apoptosis of H9c2 cardiomyocytes were assessed by DAPI and TUNEL staining. (A) Representative images of TUNEL-positive cells (green, first row) and DAPI counterstaining (blue, middle row). Scale bar: 50 μm; (B) The histogram shows the relative proportion of TUNEL-positive cells in experimental groups. Values are mean ± S.D (n = 5). **: p < 0.01 vs. control group; #: p < 0.05 vs. H/R group; ##: p < 0.01 vs. H/R group.