Figure 5.
Expression of some virulence-associated genes of R. solanacearum were quantified by qRT-PCR treated with or without coumarins. R. solanacearum was cultured in B medium treated with DMSO or the coumarins using the IC50 concentration (coumarin 58.09 mg/L, umbelliferone 37.15 mg/L, esculetin 24.15 mg/L and daphnetin 8.73 mg/L). SerC was used as the reference gene to normalize the gene expression using the ∆∆Cq method. The results reflect three biological replicates and error bars indicate the standard deviation. (* indicated p < 0.05)