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Fluorescence microscope images of biofilm formation of R. solanacearum cells after treatment with: (A) sterile water; (B) DMSO; (C,D) coumarin at final concentration of 64 μg/mL and 128 μg/mL respectively; and (E,F) carvacrol at final concentration of 64 μg/mL and 128 μg/mL, respectively. R. solanacearum were incubated with different treatment for 12 h at 30 °C without shaking, and exopolysaccharide matrixes were stained with FITC-ConA. Each treatment was repeated in triplicate.
