Figure 3.

Capsaicin-suppressed POU3F2 and shRNA-mediated knockdown of POU3F2 reduces tNOX expression and suppresses the growth of AGS cancer cells. (A) Cells were exposed to capsaicin, and cell lysates were separated by SDS-PAGE and analyzed by Western blotting. β-actin was used as an internal control; (B) Cells were transfected with shRNA-targeted POU3F2 for 24 h and harvested for protein analyses. Cell lysates were separated by SDS-PAGE and analyzed by Western blotting. β-actin was used as an internal control. The mRNA levels of POU3F2 and tNOX were determined by RT-PCR using β-actin as an internal control; (C) AGS cell growth was dynamically monitored using impedance technology. Normalized cell index (CI) values measured over 72 h are shown. con., control.