Figure 4.

The overexpression of POU3F2 enhances tNOX expression and increases the growth of AGS cancer cells. (A) Cell lysates were separated by SDS-PAGE and analyzed by Western blotting. GAPDH was used as an internal control; (B) AGS cells were seeded at least in triplicate, and the number of viable cells was determined by trypan blue exclusion assays. The presented values (mean ± SD) represent three independent experiments performed in at least triplicate (* p < 0.05, *** p < 0.001 for experimental groups vs. controls); (C) AGS cell growth was dynamically monitored using impedance technology. Normalized CI values measured over 72 h are shown.