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. 2016 Jun 4;21(6):733. doi: 10.3390/molecules21060733

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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The overexpression of POU3F2 increases AGS cell migration and invasion. (A) Dynamic monitoring of AGS cell migration was performed using impedance technology. Normalized CI values obtained over 20 h are presented; (B) Cell migration and invasion were measured by Boyden chamber system. Values (mean ± SD) are from at least three independent experiments. Cell migration was significantly enhanced in POU3F2-overexpressing cells compared to controls (* p < 0.05). Cell invasion was significantly increased in POU3F2-overexpressing cells compared to controls (** p < 0.01); (C) Cell lysates were separated by SDS-PAGE and analyzed by Western blotting. β-actin was used as an internal control.

The overexpression of POU3F2 increases AGS cell migration and invasion. (A) Dynamic monitoring of AGS cell migration was performed using impedance technology. Normalized CI values obtained over 20 h are presented; (B) Cell migration and invasion were measured by Boyden chamber system. Values (mean ± SD) are from at least three independent experiments. Cell migration was significantly enhanced in POU3F2-overexpressing cells compared to controls (* p < 0.05). Cell invasion was significantly increased in POU3F2-overexpressing cells compared to controls (** p < 0.01); (C) Cell lysates were separated by SDS-PAGE and analyzed by Western blotting. β-actin was used as an internal control.