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. 2016 Aug 5;21(8):1021. doi: 10.3390/molecules21081021

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of SOE dose on the motility of endometrial cancer cells. (A) HEC-1A cells; (B) RL95-2 cells; and (C) closure rates of both cell lines. Cancer cells were cultivated with a Culture Insert-2 Well (ibidi GmbH, Munich, Germany) for 24 h. After the culture insert was removed, DMEM with specified SOE concentrations was added, with or without TGFβ1 (2.5 ng/mL) stimulation, and the cells were then incubated. Photomicrographs of culture plates were directly obtained using a phase-contrast microscope (magnification 20×). Closure rate is defined as 100% without TGFβ1 induction and without SOE treatment. Three independent experiments were conducted; results are expressed as mean ± SD. A significant difference from the respective vehicle, according to Student’s t-test, was indicated as “a” for p < 0.05; “b” for p < 0.01, and “c” for p < 0.001.

Effects of SOE dose on the motility of endometrial cancer cells. (A) HEC-1A cells; (B) RL95-2 cells; and (C) closure rates of both cell lines. Cancer cells were cultivated with a Culture Insert-2 Well (ibidi GmbH, Munich, Germany) for 24 h. After the culture insert was removed, DMEM with specified SOE concentrations was added, with or without TGFβ1 (2.5 ng/mL) stimulation, and the cells were then incubated. Photomicrographs of culture plates were directly obtained using a phase-contrast microscope (magnification 20×). Closure rate is defined as 100% without TGFβ1 induction and without SOE treatment. Three independent experiments were conducted; results are expressed as mean ± SD. A significant difference from the respective vehicle, according to Student’s t-test, was indicated as “a” for p < 0.05; “b” for p < 0.01, and “c” for p < 0.001.