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. 2016 Aug 5;21(8):1021. doi: 10.3390/molecules21081021

Figure 4.

Figure 4

Figure 4

Effects of SOE dose on migration of endometrial cancer cells. (A) HEC-1A cells; (B) RL95-2 cells; and (C) the migration index of both cell lines. The migration assay was conducted using the Boyden chamber. HEC-1A and RL95-2 cells were treated with various doses of SOE for 48 h. Cells that penetrated the polycarbonate membrane to lower surface of the filter were stained with 0.5% crystal violet. The cell numbers were visualized under an inverted microscope with a magnification of 100× and were counted per field. The migration index is defined as 100% without TGFβ1 induction and without SOE treatment. Three independent experiments were performed; results are expressed as mean ± SD. A significant difference from the respective vehicle, according to Student’s t-test, was indicated as “a” for p < 0.05, “b” for p < 0.01, and “c” for p < 0.001.