Figure 1.

Schematic of enzyme reaction and immobilization. (A) Recombinant CrCLH1 catalyzed Chl-a to Chlide-a. The Mg ion bond with Chlide-a was unstable under low pH conditions and was followed by Mg dechelation (B) Reaction of the (3-aminopropyl) triethoxysilane (APTES) coating with magnetite nanoparticles used to immobilize recombinant CrCLH1 on the magnetic iron oxide nanoparticles (MIONPs). N-ethyl-N-3-dimethylaminopropyl carbodiimide (EDC) and N-hydroxysuccinimide (NHS) were used to form activating carboxyl groups for reaction with other amine-containing molecules.