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. 2016 Jul 26;21(8):972. doi: 10.3390/molecules21080972

Figure 3.

Figure 3

Capacity of APTES-coated MIONPs to immobilize the crude cell lysate with recombinant CrCLH1. (A) Schematic representation of the recombinant CrCLH1; (B) SDS-PAGE and western blot analysis of the crude cell lysate before (Lane 1) and after (Lane 2) enzyme immobilization. Western blot analysis was performed using a monoclonal anti-His tag antibody. M: marker; Lane 1: crude cell lysate; Lane 2: crude cell lysate after binding. The arrow indicates the location of recombinant CrCLH1. The molecular mass of the recombinant CrCLH1 was 40.35 kDa. The protein gel was stained with Coomassie blue.