Table 1.

Characterization of the free and immobilized CrCLH1.

Samples	Amount of Protein Binding (mg/g Gel)	Immobilization Yield (% b)	Enzymatic Activity (U c/g Gel)	Specific Activity of Immobilized Enzymes (U/mg Protein)	Specific Activity of Non-Immobilized Enzymes (U/mg Protein)
Free CrCLH1	0	ND a	ND	ND	186 ± 22
Immobilized CrCLH1	32.1 ± 0.3	98.99 ± 0.91	722.3 ± 50.3	131.0 ± 9.1	4.1 ± 0.3

^a Not detected; ^b Immobilization yield (%) was defined as follows: Immobilization yield (%) = [Total enzyme − nonimmobilized enzyme (mg)/Total enzyme (mg)] × 100; ^c The enzyme activity was determined by measuring the absorbance of the aqueous EtOH phase with Chlide-a at 667 nm. One unit of Chlase activity is defined as described in the Experimental Section.