Figure 3.

Neuroprotection exerted by Ch-PRE in SK-N-MC cells: (A) neuroprotective effect of Ch-PRE. Cell viability is expressed as percentage of viable cells. SK-N-MC cells were pre-incubated with 0.25, 0.5 and 1 µg GAE·mL⁻¹ of Ch-PRE for 24 h and then injured with 300 µM H₂O₂ for 24 h. Control represents cells non-challenged with stress inducers or with Ch-PRE; Stress represents cells challenged with stress inducer and not treated with Ch-PRE. Statistical differences between control or treated cells and stressed cells are denoted as ** p < 0.01, *** p < 0.001 and statistical differences between treatments and control (not-treated cells) are denoted as # p < 0.05 and ### p < 0.001; (B) Relative ROS production by SK-N-MC cells pre-incubated with Ch-PRE for (B1) 2 h and (B2) 24 h and submitted to an oxidative stress (300 µM H₂O₂ for 30 min). Statistical differences in relation with cells not treated are denoted as *** p < 0.001. All values are means of three independent experiments ± SD.