Table 1.
Results of the quantitative fluorescent assay for simultaneous identification of apoptotic and necrotic cells due to the loss of membrane integrity in Candida albicans ATCC 10231 treated with oleuropein in vitro for 18 h.
| Sample | Viable Cells (%) | Non-Viable Cells | ||
|---|---|---|---|---|
| Σ | Apoptosis (%) | Necrosis (%) | ||
| OLP1 | 16.3 ± 2.1 | 83.7 ± 2.1 OLP2,OLP3,NC | 39.3 ± 11.2 OLP2,OLP3,NC,PC | 44.3 ± 10.2 OLP2,OLP3,NC,PC |
| OLP2 | 61.3 ± 6.0 | 38.7 ± 6.0 OLP3,NC,PC | 29.0 ± 4.6 OLP3,NC,PC | 9.7 ± 1.5 NC |
| OLP3 | 76.0 ± 6.6 | 24.0 ± 6.6 NC,PC | 14.7 ± 5.5 NC,PC | 9.3 ± 1.5 NC |
| PC | 11.7 ± 3.2 | 88.3 ± 3.2 NC | 78.7 ± 0.6 NC | 9.7 ± 3.8 NC |
| NC | 97.3 ± 0.6 | 2.7 ± 0.6 | 1.0 ± 1.0 | 1.7 ± 1.1 |
Three hundred cells per sample per each experimental point were analyzed. Mean values ± SD are shown. OLP—concentration of oleuropein (OLP1—12.5 mg·mL−1, OLP2—1.25 mg·mL−1, OLP3—0.195 mg·mL−1); PC—positive control; NC—negative control (RPMI). Statistical significance of data was evaluated using a χ2 test. The level of statistical significance was set at p < 0.05. The abbreviations next to the means indicate from which groups the relevant group differs with statistical significance.