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The effect of thymol on the generation H2O2 and O2•− in the leaves of tobacco seedlings under Cd stress. The roots of seedlings were treated with water (control), CdCl2 (20 μM), CdCl2 (20 μM) + thymol (100 μM), and thymol (100 μM) for 72 h. After treatment, the seedlings were excised at the base of the stem and supplied DAB or NBT for 6 h through the cut stems. Then the leaves were decolorized with ethanol and photographed with a stereoscopic microscope. Bar = 1 mm.
