Figure 5.

The effect of thymol on cell death in tobacco seedlings under Cd stress. The roots of seedlings were treated with water (control), CdCl₂ (20 μM), CdCl₂ (20 μM) + thymol (100 μM), and thymol (100 μM) for 72 h. (A) The roots were loaded with PI and photographed with a fluorescent microscope; (B) The roots were stained with trypan blue and photographed with a stereoscopic microscope; (C) The seedlings were excised at the base of the stem and supplied trypan blue for 6 h through the cut stems. Then the leaves were decolorized with ethanol and photographed with a stereoscopic microscope. Bar = 1 mm.