Figure 7.

The effect of thymol on GSH content in tobacco seedlings under Cd stress. The roots of seedlings were treated with water (control), CdCl₂ (20 μM), CdCl₂ (20 μM) + thymol (100 μM), and thymol (100 μM) for 72 h. Then the roots were loaded with monochlorobimane for the observation of green fluorescence (A) and the quantification of fluorescent density (B). Bar = 1 mm. The GSH content was determined by in-tube assay in roots (C) and leaves (D), respectively. Different letters in (B–D) indicate that the mean values of three replicates are significantly different between the treatments (p < 0.05, ANOVA, LSD).