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. 2016 Jul 11;21(7):895. doi: 10.3390/molecules21070895

Figure 3.

Figure 3

Conditions for the covalent immobilization of ST2570CQ on SBA-15. (A) The optimization of pH for the immobilization system using either, 50 mM PB buffer (pH 6.0–9.0) or 50 mM Gly-NaOH buffer (pH 8.6–10.0); (B) The optimization of the initial amount of enzyme per 10 mg support for immobilization system; (C) The optimization of temperature for immobilization system; (D) The optimization of time for the immobilization. The immobilization system consisted of 2 mL 50 mM PB buffer (pH 6.0) containing 10 mg support in a 20 mL reactor. We set the best enzyme activity as 100%. Three trials were performed for each group.