. 2016 Apr 25;21(5):398. doi: 10.3390/molecules21050398

Table 4.

Heterologous, degenerate and specific primers used for isolating cDNAs from cherimoya cv. “Concha Lisa”, RACE-PCR and qPCR assays.

Primer Name	Forward (F) and Reverse (R) Primers (5′→3′)	T_m (°C)	Reference
PpNADP-IDH heterologous primers (Prunus persica)	F: GGCCATGTACAACACTGATGAG	58	Yang et al. [43]
PpNADP-IDH heterologous primers (Prunus persica)	R: CATTGTCATCCAACTTTGCCCTG	58	Yang et al. [43]
AcNADP-IDH	F: TCCAAATGGAAGTCAAAGTTCGAG	64	N/A ¹
AcNADP-IDH	R: CCTTTCTGGTGAACACGGTAATG	64	N/A ¹
CmACON heterologous primers (Citrus maxima)	F: GAAGCAATCACCAAAGGGAATC	52	Yang et al. [43]
CmACON heterologous primers (Citrus maxima)	R: TACTACGGTGAATTCGCTCAAAG	52	Yang et al. [43]
AcACON RACE-PCR primers	F: ACCATGTCCCCTCCTGGCCC	64	N/A
AcACON RACE-PCR primers	R: TCCACATTGGATTTCCTTTCGTG	64	N/A
AcACON qPCR primers	F: GCAGGCACGGTTGACATTGATT	64	N/A
AcACON qPCR primers	R: GCCTGATGGCACGGATAATTGG	64	N/A
VvNADP-ME heterologous primers (Vitis vinifera)	F: GGAGGAGTTCGTCCTTCAGCCTG	58	Yang et al. [43]
VvNADP-ME heterologous primers (Vitis vinifera)	R: CCTTTGAGTCCACAAGCCAAATC	58	Yang et al. [43]
AcNADP-ME RACE-PCR primers	F: GCACAATCTCCGCCAATATGAAG	64	N/A
AcNADP-ME RACE-PCR primers	F: AAGCCTCGCCAACGGTCGGT	64	N/A
AcNADP-ME qPCR primers	F: AGAACGACTGGTCAGGAGTATG	64	N/A
AcNADP-ME qPCR primers	R: CCTCGCCTGCGCCAAGGAATA	64	N/A
VvNAD-ME heterologous primers (Vitis vinifera)	F: TGATCTTGGAGTTCAGGGAATTGGA	58	Yang et al. [43]
VvNAD-ME heterologous primers (Vitis vinifera)	R: GCACTTCCTGCTCCAACTACAACTA	58	Yang et al. [43]
AcNAD-ME	F: GCTGCTGGCATGAATCCACAAA	64	N/A ¹
AcNAD-ME	R: GCGTGTAAAAACAGCTTCCATGA	64	N/A ¹
PEPC degenerate primers	F: CCITGGATHTTYGCITGGAC	54	Moing et al. [44]
PEPC degenerate primers	R: GCIGCDATICCYTTCATIG	54	Moing et al. [44]
AcPEPC RACE-PCR primers	F: GAGACCCTGGTATTGCTGCTTT	64	N/A
AcPEPC RACE-PCR primers	R: ACATTTTCTTGAGCATTTGGAGC	64	N/A
AcPEPC qPCR primers	F: TGGACCCAGACCAGATTTCACC	66	N/A
AcPEPC qPCR primers	R: AATTGCTTCTCAGCCGCTCACC	66	N/A
mCS degenerate primers	F: GGTTTRGCTGGRCCACTYC	46	Iannetta et al. [14]
mCS degenerate primers	R: GRGCATCAACATTWGGC	46	Iannetta et al. [14]
Ac-mCS	F: ATCAAGTCTGTTGTAGAAGAATGT	62	N/A ¹
Ac-mCS	R: CTCTGGCATGTGTATCGTGGA	62	N/A ¹
AcATP-CS	F: AAGTTCACCGTCCTCAACCCTA	62	González-Agüero et al. [45]
AcATP-CS	R: GCATAACCCAAATCTCCAACAG	62	González-Agüero et al. [45]
DEGcyNAD-MDH degenerate primers	F: TTTCYATYTACAAGTCMCARGC	46	N/A ²
DEGcyNAD-MDH degenerate primers	R: CAGCTTWCGRGCCTTGATRATT	46	N/A ²
Ac-cyNAD-MDH	F: AGAGTTTGCTCCATCCATTCCT	62	N/A ¹
Ac-cyNAD-MDH	R: CTCGTTGTTGGACGGTTGTAAT	62	N/A ¹
AcUbiq (Genbank FJ664263)	F: TCCTGCAGAATCAGTGGAGTC	64	González-Agüero et al. [45]
AcUbiq (Genbank FJ664263)	R: AGGAACCAAATCCGCAAACAGC	64	González-Agüero et al. [45]

¹ Specific primer for A. cherimola designed for RACE-PCR and also used for qPCR assays; ² Degenerate primers designed using conserved domain by a ClustalW alignment from sequences of several plant NAD-MDHs.