Table 1.
Validation parameters (n = 5).
| Parameter | Daidzein | Genistein | Formononetin | Coumestrol |
|---|---|---|---|---|
| Concentration range (μg·mL−1) | 1.0–50.0 | 0.01–30.0 | 0.01–5.0 | 0.01–10.0 |
| Regression equation | y = 396815x + 104631 | y = 398812x − 155217 | y = 509672x − 245893 | y = 118314x − 67144 |
| Correlation coefficient R | 0.9998 | 0.9999 | 0.9999 | 0.9999 |
| Precision (* RSD %) | 0.5–1.1 | 0.6–1.2 | 0.6–1.0 | 0.5–1.3 |
| Accuracy (mean recovery %) | 98.72 | 98.91 | 97.58 | 98.11 |
| LOD (ng·mL−1) | 18.2 | 25.0 | 28.1 | 27.4 |
| LOQ (ng·mL−1) | 60.7 | 83.3 | 93.7 | 91.3 |
* RSD—relative standard deviation.