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. 2016 Nov 4;21(11):1469. doi: 10.3390/molecules21111469

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of BCF on H/R induced myocardial apoptosis. H9c2 cells were pretreated with the indicated BCF concentrations for 4 h followed by 6 h of hypoxia and 12 h of reoxygenation or pretreated with indicated BCF concentrations for 24 h before the detection of relevant indexes. (A) Cytotoxicity was detected by CCK-8 assay; (B) Cell viability was determined by CCK-8 assay; (C) The apoptotic ratio of H9c2 cardiomyocytes was measured by flow cytometry using Annexin V-FITC and PI staining; (D) The apoptosis rate was quantified by BD FACS software; (E) Caspase-3 and cleaved caspase-3 was detected by western blotting. Values were represented as mean ± SD (n = 6, each group). ^## p < 0.01 vs. normal; ** p < 0.01 vs. H/R-treated cells.