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. 2016 Nov 17;21(11):1520. doi: 10.3390/molecules21111520

Figure 6.

Figure 6

Effect of Sin and 1a–2f on the LPS-induced mRNA expression of the inflammatory factors IL1β, IL6, and TNF-α in Raw264.7 cells. Raw264.7 cells were treated concurrently with 1 µg/mL of LPS (blank was untreated) and 10 µg/mL of Sin or 1a–2f for 12 h. The cells were washed, the mRNA was extracted using Trizol, and the mRNA expression was determined using RT-PCR. In general, the derivatives enhanced the Sin induced IL1β and TNF-α inhibition, while reducing the IL6 inhibition. Results are expressed as mean ±SD (n = 3). * p < 0.05 vs. vehicle (model group); # p < 0.05 vs. Sin.