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. 2016 Feb 27;21(3):277. doi: 10.3390/molecules21030277

Figure 4.

Figure 4

Inhibition of GR transactivation by L. obtusiloba extracts. Cortisol-induced GR transactivation in HeLa cells (a). After HeLa cells were transfected with the pGRE-Luc 12 h before cortisol treatment. Construct-deleted 5xGRE DNA fragment (pΔGRE-Luc) was used as a negative control. *** p < 0.001 vs. pΔGRE-Luc. Inhibition of GR transactivation by RU 486 (b). pGRE-Luc was transfected into HeLa cells, and the transfected cells were treated with the indicated doses of RU 486 3 h before stimulation with 500 nM cortisol. * p < 0.05 and ** p < 0.01 vs. non-treatment (+cortisol). Inhibition of GR transactivation by L. obtusiloba extracts (c). pGRE-Luc was transfected with the pGRE-Luc into HeLa cells, and the transfected cells were treated with the indicated doses of L. obtusiloba extracts 3 h before stimulation with 500 nM cortisol. * p < 0.05 and ** p < 0.01 vs. non-treatment (+cortisol). Results are normalized for transfection efficiency and plotted as arbitrary units (A.U) in triplicate samples from three independent transfections. Effect of L. obtusiloba extracts on HeLa cell viability (d). Viability of cells was assessed by WST-1 assay. All results are expressed as mean ± SEMs.