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Determination of the buffering capacity of PEI 25 kDa, P123-PEI-R4, P123-PEI-R11 and P123-PEI-R18 by acid-base titration. Each polymer (6 mg) was first dissolved in 30 mL water, and subsequently set to pH 10.0 using 0.1 M NaOH. Then 25 µL increments of 0.1 M HCl were added, and the pH of the solution was measured with a pH meter after each addition. The solutions were titrated to about pH 3.0 with 0.1 M HCI. The titration curve of water is presented as a control.
