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. 2016 May 18;21(5):655. doi: 10.3390/molecules21050655

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Green fluorescent protein (GFP) reporter gene transfection in B16 cells by PEI 25 kDa (a); P123-PEI (b); P123-PEI-R4 (c); P123-PEI-R11 (d) and P123-PEI-R18 (e) in 24-well plate. Note: 100 µL polymer/DNA complex solution containing 2.5 µg plasmid pEGFP-N2 at various weight ratios ranging from 5 to 30 were added to each well; (B) Transfection efficiency of PEI 25 kDa/DNA, P123-PEI/DNA, P123-PEI-R4/DNA, P123-PEI-R11/DNA and P123-PEI-R18/DNA in B16 cells at the polymer/pDNA w/w ratio of 5, 10, 20, and 30 in the 24-well plate. Note: 100 µL polymer/DNA complex solution containing 2.5 µg plasmid pGL3-Control at various weight ratios ranging from 5 to 30 was added to each well.