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Hydrolysis of substrates 1 and 5 at different concentration.
| Substrate | Conc. (mM) | t (h) | Vh 1 | Conversion (%) (SD) | Product (Yield %) 5′-OH (SD) |
|---|---|---|---|---|---|
| 1 | 10 | 24 | 0.48 | 94 (5) | 1a (94) (5) |
| 1 | 20 | 48 | 0.30 | 92 (4) | 1a (86) (6) |
| 1 | 40 | 72 | 0.28 | 90 (8) | 1a (79) (10) |
| 5 | 10 | 48 | 0.09 | 94 (3) | 5a (66) (7) |
| 5 | 40 | 72 | - | 88 (9) | 5a (85) (6) |
Experimental conditions: 10% CH3CN in 25 mM phosphate buffer pH 7, agarose CNBr-protease N 6 IU, reaction volume 5 mL, r.t., SD = standard deviation.
