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PCR amplification carried out using saffron and curcuma markers on saffron-curcuma artificial mixtures. (A) We show the amplification carried out using the crocus-specific marker; (B) We show the amplification carried out using the curcuma-specific marker. As expected, the amplification is present just in crocus, curcuma and in the mixtures. The ratio (such an 80/20) refers to the different percentages of saffron and curcuma powders mixed before the DNA extraction.
