Table 2.
Details of species-specific primer pairs used for the amplifications in this study. Universal primers are divided from the primers developed in the present study with a line.
| Primer Name a | Sequence | Annealing Temperature | Amplicon Size (bp) | Typology |
|---|---|---|---|---|
| matK-KIM1R | ACCCAGTCCATCTGGAAATCTTGGTTC | 58 °C | Variable, circa 900 | Universal |
| matK-KIM3F | CGTACAGTACTTTTGTGTTTACGAG | |||
| rbcL-F | ACCACAAACAGAGACTAAAGC | 52 °C | Variable circa 600 | |
| rbcL-R | GTAAAATCAAGTCCACCRCG | |||
| ITS-S2F | ATGCGATACTTGGTGTGAAT | 52 °C | Variable circa 400 | |
| ITS4 | TCCTCCGCTTATTGATATGC | |||
| Gard_matK_Fw | TGGGATACTCTTATTGATAG | 55 °C | 391 | Primers developed in the present study |
| Gard_matK_Rev | CCGGGTGAAACCAAATAC | |||
| Budd_matK_Fw | GAACGTCTTTGTTAAGGTTAAG | 58 °C | 189 | |
| Budd_matK_Rev | CTTGGATGAAACCAAAGCGA | |||
| Curc_matK_Fw | GTAAAAATAGAACATCTTGGAG | 56 °C | 202 | |
| Curc_matK_Rev | ATATGGTTGAGACCAAAAATG | |||
| Cart_matK_Fw | TGTATGTGAATATGAATCTGGC | 54 °C | 387 | |
| Cart_matK_Rev | CCATTGAACGCTTTACCGCG | |||
| Croc_matK_Fw | ATCTTATAATAGTATGTTGTGAT | 54 °C | 192 | |
| Croc_matK_Rev | TGTATGATTGATACCAAAAGT | |||
| Cal_matK_Fw | CATACTCTGGGCCACAAC | 53 °C | 435 | |
| Cal_matK_Rev | GAGGAAGCCGTATTCATATT |
a Gard, gardenia; Budd, buddleia; Curc, curcuma; Cart, Carthamus; Croc, crocus; Cal, calendula.