Table 3.
Results of the amplificability tests with the universal primers for plant barcoding on the extractions carried out with the three methods.
| Sample | Extraction Methods | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| GeneElute Plant | Plant DNA Purification | DNeasy Plant | |||||||
| matK | rbcL | ITS | matK | rbcL | ITS | matK | rbcL | ITS | |
| >900 a | >600 | >400 | >900 | >600 | >400 | >900 | >600 | >400 | |
| AD.01.JM | + b | + | + | / | / | / | / | / | / |
| AD.02.JM | + | + | + | / | / | / | / | / | / |
| AD.03.JM | / | / | / | / | / | / | / | / | / |
| AD.04.POL | + | + | + | + | + | + | + | + | + |
| AD.05.POL | / | / | / | / | / | / | / | / | / |
| AD.06.POL | + | + | + | + | + | + | + | + | + |
| AD.07.POL | + | + | + | / | / | / | +/− | +/− | +/− |
| AD.08.POL | + | + | + | / | / | / | / | / | / |
| AD.09.POL | / | / | / | / | / | / | / | / | / |
| AD.10.POL | / | / | / | / | / | / | / | / | / |
| AD.11.JM | + | + | + | / | / | / | / | / | / |
| AD.12.JM | + | + | + | / | / | / | / | / | / |
| AD.13.JM | + | + | + | / | / | / | / | / | / |
| AD.14.JM | / | / | / | / | / | / | / | / | / |
| AD.15.BM | + | + | + | / | / | / | / | / | / |
| AD.16.BM | + | + | + | / | / | / | / | / | / |
| AD.17.BM | + | + | + | / | / | / | / | / | / |
| AD.18.BM | + | + | + | + | + | + | + | + | + |
| AD.19.BM | + | + | + | + | + | + | + | + | + |
| AD.20.BM | + | + | + | + | + | + | + | + | + |
| AD.21.BM | + | + | + | + | + | + | + | + | + |
| AD.22.JM | + | + | + | / | / | / | / | / | / |
a expected size of the corresponding amplicons in base pairs; the expected size is higher than: 900 bp for matK; 600 bp for rbcL and 400 bp for the internal transcribed spacer (ITS). b a positive result or the absence of amplification are reported respectively with “+” and “/”. +/− refers to samples with a very weak amplification.