Table 1.

Antiproliferative activity of N′-(4-arylidene)-1H-benzo[d]imidazole-2-carbohydrazides 3–19.

Compd.	Ar		IC50 (μM) a
		L1210	CEM	HeLa	Mia-Paca-2
3	2-OH-phenyl	5.7 ± 0.9	4.4 ± 0.2	12 ± 0.7	34 ± 0.9
4	3-OH-phenyl	94 ± 7	98 ± 10	88 ± 12.5	>100
5	4-OH-phenyl	47 ± 2	85 ± 0.01	>100	>100
6	2,4-(OH)2-phenyl	2.6 ± 0.9	2.6 ± 1.0	4.7 ± 1.6	21 ± 12
7	2,5-(OH)2-phenyl	22 ± 7	4.9 ± 1.2	77 ± 18	>100
8	2,3,4-(OH)3-phenyl	47 ± 7	20 ± 8	97 ± 28	>100
9	2,4,6-(OH)3-phenyl	90 ± 7	>100	>100	>100
10	2-OH-4-OMe-phenyl	1.6 ± 0.9	0.98 ± 0.02	4.0 ± 0.4	6.3 ± 3.2
11	2-OH-3-OEt-phenyl	5.9 ± 2.4	6.3 ± 1.9	22 ± 0.02	23 ± 8
12	3-OH-4-OMe-phenyl	92 ± 4	57 ± 3.7	>100	>100
13	2-OH-4-N(Et)2-phenyl	14 ± 2	4.8 ± 0.8	23 ± 3	40 ± 6
14	2-OH-5-Cl-phenyl	7.4 ± 2.5	1.8 ± 0.6	4.8 ± 0.9	9.2 ± 4.0
15	2-OH-5-Br-phenyl	5.0 ± 2.8	1.8 ± 0.9	4.9 ± 0.4	35 ± 5
16	2-OH-naphtyl	2.9 ± 1.3	1.0 ± 0.01	2.5 ± 1.4	7.9 ± 0.3
17	phenyl	>250	>250	>250	220 ± 37
18	4-OMe-phenyl	>250	>250	>250	>250
19	napht-1-yl	240±13	>250	>250	>250

^a IC₅₀ values (compound concentration that reduces cell growth by 50%) are shown.