Table 2.
Structures and cytotoxicity of compounds 13a–l against HT-29, H-460, A-549, and MDA-MB-231 cells in vitro.
| Compd. | R1 | IC50 (μM) a | |||
|---|---|---|---|---|---|
| HT-29 | H-460 | A549 | MDA-MB-231 | ||
| 13a | H | 30.52 ± 2.56 | 28.64 ± 1.08 | 44.83 ± 2.42 | 32.16 ± 1.59 |
| 13b | 2,4-diCH3 | 45.08 ± 1.37 | NA | NA | NA |
| 13c | 2-Cl, 6-CH3 | 12.95 ± 0.68 | 24.66 ± 0.80 | 30.71 ± 1.73 | 25.42 ± 1.14 |
| 13d | 2,6-diCH3 | 18.37 ± 0.45 | 24.62 ± 2.75 | 15.94 ± 0.95 | 28.12 ± 2.44 |
| 13e | 2,6-diF | 35.10 ± 2.81 | ND | NA | 20.94 ± 1.63 |
| 13f | 3-Cl, 4-F | 6.33 ± 0.93 | 15.28 ± 1.19 | 10.49 ± 2.26 | 12.75 ± 2.97 |
| 13g | 3-Cl | 10.07 ± 0.32 | 5.86 ± 1.34 | 15.43 ± 1.02 | 21.95 ± 2.58 |
| 13h | 3-CF3 | ND | ND | ND | ND |
| 13i | 4-OCF3 | 26.58 ± 1.27 | 23.46 ± 2.29 | 16.54 ± 0.70 | 30.65 ± 3.06 |
| 13j | 4-F | 17.95 ± 0.94 | 32.28 ± 2.87 | ND | NA |
| 13k | 3-F | 30.50 ± 3.16 | ND | NA | NA |
| 13l | 2-F | 24.18 ± 1.35 | 32.29 ± 2.63 | NA | 35.56 ± 2.85 |
| Sorafenib | 3.27 ± 0.32 | 2.15 ± 0.43 | 4.47 ± 0.28 | 3.81 ± 0.50 | |
a Results are expressed as means ± SD (standard deviation) of three independent experiments. NA: compound showing IC50 value > 50 μM. ND: Not determined.