Table 5.
The Box-Behnken experimental design and experimental variables (X1: pressure; X2: temperature; X3: ethanol content) and responses (Y1: major flavonol content; Y2: IC50 value).
| Run | X1 (MPa) | X2 (°C) | X3 (Vethanol/Vwater, %) | Y1 (mg/g) | Y2 (mg/mL) |
|---|---|---|---|---|---|
| 1 | 30 | 50 | 60 | 34.79 | 0.447 |
| 2 | 30 | 40 | 75 | 33.56 | 0.357 |
| 3 | 20 | 40 | 60 | 32.48 | 0.435 |
| 4 | 20 | 50 | 75 | 40.33 | 0.305 |
| 5 | 30 | 60 | 75 | 36.11 | 0.407 |
| 6 | 20 | 50 | 75 | 39.92 | 0.319 |
| 7 | 20 | 50 | 75 | 40.51 | 0.296 |
| 8 | 20 | 60 | 60 | 34.19 | 0.391 |
| 9 | 20 | 50 | 75 | 39.97 | 0.314 |
| 10 | 10 | 40 | 75 | 32.13 | 0.315 |
| 11 | 20 | 40 | 90 | 37.52 | 0.302 |
| 12 | 20 | 50 | 75 | 40.72 | 0.281 |
| 13 | 10 | 60 | 75 | 35.74 | 0.299 |
| 14 | 20 | 60 | 90 | 41.58 | 0.304 |
| 15 | 10 | 50 | 90 | 38.43 | 0.311 |
| 16 | 30 | 50 | 90 | 38.98 | 0.309 |
| 17 | 10 | 50 | 60 | 33.41 | 0.327 |