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. 2016 Apr 30;21(5):574. doi: 10.3390/molecules21050574

Figure 5.

Figure 5

Celastrol regulates the expression of the C-KIT/AML-ETO oncoproteins and downstream signaling pathways. (A) The expression of C-KIT in Kasumi-1 cells treated with celastrol for 24 h was analyzed using real-time quantitative RT-PCR; (B) Celastrol downregulated the C-KIT and AML1-ETO protein levels; (C,D) Effects of celastrol on AKT/STAT3/Erk pathway in Kasumi-1 cells. Cells were treated with celastrol as indicated, and the expression of kinases were evaluated using western blotting; (E) Kasumi-1 cells were incubated with 40 μM LY294002 and 1.0 μM celastrol for 24 h. Cell viability was analyzed using the CCK-8 assay. (F) Kasumi-1 cells were transiently transfected with constitutively active AKT plasmid (AKT) and vector control (V) by electroporation. After 24 h, cells were exposed to celastrol (2.5 μM) for another 24 h. Cell viability was analyzed using the CCK-8 assay. Data are from three independent experiments. * p < 0.05, and ** p < 0.01.