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Effect of TXA1·HCl on the colony forming efficiency of MCF-7 cells. Cells were treated for 6 or 24 h with 5, 10 and 20 μM TXA1·HCl. Results were analyzed 14 days after recovery of the cells previously treated with TXA1·HCl. Representative images (a) and analysis of the number of colonies (b). Results are expressed as mean ± SEM, from three independent experiments. * p ≤ 0.05; *** p ≤ 0.001; **** p ≤ 0.0001 when comparing treatment vs. control (H2O).
