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Binding of DSF to C. vulgaris urease at equilibrium. The change in the intrinsic fluorescence (ΔIF) was measured an hour after the addition of the indicated concentrations of DSF. A non-linear curve fit to Equation (5) was made and is indicated by the solid grey line. Curve data is obtained in one of three identical binding saturation experiments. Non-linear curve fitting and plots were prepared using Graph Pad Prism 5®.
