Table A1.

Residues detected as putative HS on hsGS PPI and their classification as HS, WS or NS. In the columns “A” to “J”, each one referring to a GS chain, an x indicates that the residue on that monomer was detected as a potential HS (buried area ≥ 40 Ų). If the residue was detected in at least 6 monomers, ΔΔG_bind was determined by cASM and his classification (HS/WS/NS) is presented in the last column.

Resid	A	B	C	D	E	F	G	H	I	J	Tested	Classif
10		x			x	x	x	x		x	no
11	x				x	x	x		x	x	no
13		x	x	x	x	x	x	x	x	x	yes	WS
14	x		x		x	x	x		x	x	yes	WS
15	x				x	x	x		x		no
16		x		x		x	x		x	x	no
17	x	x	x	x	x	x	x	x	x	x	yes	WS
18	x	x		x	x	x	x		x	x	yes	NS
20	x	x	x	x	x	x	x	x	x	x	yes	HS
22	x	x	x	x	x	x	x	x	x	x	yes	NS
25								x			no
27		x	x	x	x	x	x	x	x	x	yes	HS
41	x	x	x	x	x	x	x	x	x	x	yes	HS
42		x	x	x	x	x	x	x	x	x	yes	HS
43		x	x	x		x	x	x	x	x	yes	NS
44		x	x	x	x	x		x	x	x	yes	HS
46	x	x	x	x	x	x	x	x	x	x	yes	WS
63					x	x	x			x	no
66		x	x	x		x	x	x	x	x	yes	NS
73	x				x	x		x	x		no
74				x							no
76	x	x	x	x	x	x	x	x	x	x	yes	WS
78				x	x	x				x	no
90				x			x		x	x	no
91						x	x				no
104		x									no
142	x		x			x	x		x	x	no
143					x	x					no
145					x						no
152	x	x	x	x		x	x	x	x	x	yes	NS
154	x	x	x			x	x	x	x	x	yes	NS
162	x	x	x	x	x	x	x	x	x	x	yes	HS
163	x	x	x	x	x	x	x	x	x	x	yes	WS
165	x	x	x	x	x	x	x	x	x	x	yes	HS
168		x	x					x		x	no
173	x	x	x	x	x	x	x		x		yes	HS
174	x	x	x	x	x	x	x	x	x	x	yes	HS
177	x	x	x	x	x	x	x	x	x	x	yes	HS
180	x	x	x	x	x		x	x	x	x	yes	HS
181	x	x	x	x	x	x	x	x	x	x	yes	HS
184	x	x	x	x	x	x	x	x	x	x	yes	WS
185	x	x	x	x	x	x	x	x	x	x	yes	WS
193		x	x					x		x	no
226			x	x					x	x	no
227				x	x						no
230	x	x	x	x	x	x	x	x	x	x	yes	HS
231		x	x		x	x				x	no
232	x	x	x	x	x	x	x	x	x	x	yes	NS
235	x			x	x	x				x	no
318								x			no
319	x	x	x	x	x	x	x	x	x	x	yes	HS
324			x								no
327	x	x	x	x	x	x	x	x	x	x	yes	HS
328								x			no