Table A2.
Residues detected as putative HS on zmGS PPI and their classification as HS, WS or NS. In the columns “A” to “J”, each one referring to a GS chain, an x indicates that the residue on that monomer was detected as a potential HS (buried area ≥ 40 Å2). If the residue was detected in at least 6 monomers, ΔΔGbind was determined by cASM and his classification (HS/WS/NS) is presented in the last column.
| Resid | A | B | C | D | E | F | G | H | I | J | Tested | Classif |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 3 | x | x | x | x | x | x | x | x | x | yes | NS | |
| 4 | x | x | x | x | x | x | x | x | x | x | yes | WS |
| 5 | x | x | x | x | x | x | no | |||||
| 6 | x | x | x | x | x | x | x | x | yes | NS | ||
| 7 | x | x | x | x | x | x | x | x | x | x | yes | |
| 8 | x | x | x | x | x | x | x | x | x | x | yes | NS |
| 9 | x | no | ||||||||||
| 10 | x | x | x | x | x | x | x | x | x | x | yes | WS |
| 12 | x | x | x | x | x | x | x | x | yes | NS | ||
| 15 | x | x | x | x | x | x | x | yes | NS | |||
| 16 | x | no | ||||||||||
| 18 | x | x | x | x | no | |||||||
| 20 | x | x | x | x | x | x | x | x | x | yes | WS | |
| 32 | x | x | no | |||||||||
| 33 | x | x | no | |||||||||
| 34 | x | x | x | x | x | x | x | x | x | yes | HS | |
| 35 | x | x | x | x | x | x | no | |||||
| 36 | x | x | x | x | x | x | no | |||||
| 38 | x | no | ||||||||||
| 39 | x | x | x | x | x | x | x | x | x | x | yes | WS |
| 56 | x | x | x | x | x | x | x | x | yes | NS | ||
| 59 | x | x | x | x | x | x | x | x | x | x | yes | NS |
| 66 | x | x | x | x | x | no | ||||||
| 67 | x | x | x | x | no | |||||||
| 68 | x | no | ||||||||||
| 69 | x | x | x | x | x | x | x | x | x | yes | NS | |
| 79 | x | x | no | |||||||||
| 81 | x | no | ||||||||||
| 82 | x | x | no | |||||||||
| 83 | x | x | x | x | x | x | no | |||||
| 84 | x | x | x | x | x | x | x | x | yes | HS | ||
| 137 | x | x | x | x | x | no | ||||||
| 138 | x | x | x | x | x | no | ||||||
| 139 | x | no | ||||||||||
| 140 | x | x | no | |||||||||
| 141 | x | no | ||||||||||
| 147 | x | x | x | x | x | x | no | |||||
| 148 | x | no | ||||||||||
| 150 | x | x | x | x | x | x | x | x | yes | NS | ||
| 158 | x | x | x | x | x | x | x | x | x | x | yes | HS |
| 159 | x | x | x | x | x | x | x | x | yes | NS | ||
| 161 | x | x | x | x | x | x | x | x | x | x | yes | HS |
| 164 | x | x | x | x | x | x | x | x | x | x | yes | NS |
| 165 | x | x | x | x | x | x | no | |||||
| 169 | x | x | x | x | x | x | x | x | x | yes | HS | |
| 170 | x | x | x | x | x | x | x | x | x | x | yes | NS |
| 173 | x | x | x | x | x | x | x | yes | HS | |||
| 176 | x | x | x | x | x | x | x | x | x | yes | HS | |
| 177 | x | x | x | x | x | x | x | x | x | x | yes | WS |
| 180 | x | x | x | x | x | x | x | x | x | x | yes | WS |
| 181 | x | x | x | x | x | x | x | x | x | x | yes | WS |
| 187 | x | x | x | no | ||||||||
| 189 | x | x | x | x | x | x | no | |||||
| 190 | x | no | ||||||||||
| 193 | x | no | ||||||||||
| 201 | x | x | x | no | ||||||||
| 222 | x | x | x | x | x | x | x | x | yes | WS | ||
| 223 | x | x | x | x | x | x | x | yes | NS | |||
| 224 | x | no | ||||||||||
| 226 | x | x | x | x | x | x | x | x | x | x | yes | HS |
| 227 | x | x | x | x | x | x | x | x | x | x | yes | NS |
| 229 | x | x | x | x | x | no | ||||||
| 231 | x | x | x | x | no | |||||||
| 310 | x | x | x | x | x | x | x | yes | NS | |||
| 311 | x | x | x | x | x | x | no | |||||
| 316 | x | no | ||||||||||
| 319 | x | x | x | x | x | x | x | x | x | x | yes | HS |