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Comparison of the active sites of M. sativa CHS and R. palmatum BAS. (A) Synthesis of benzalacetone; (B) Schematic representations of the active site of BAS and CHS. The presence of Leu-208, Leu-125, and Cys-190 and the reorientation of Ser-331 in BAS changed the orientation of the starter acyl-CoA, p-coumaroyl-CoA, as well as the orientation of the growing polyketide intermediate, leading to the formation of benzalacetone.
