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. 2016 Sep 22;21(10):1272. doi: 10.3390/molecules21101272

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cellular distribution of DOX and DOX-Val in MCF-7 cells observed by CLSM. DOX and DOX-Val (10 μM) were incubated for 1 h and 3 h in MCF-7 cells. Red and blue colors indicate DOX and 4′,6-diamidino-2-phenylindole (DAPI), respectively. DAPI, DOX, merged, and 2.5 D images are presented. The 2.5 D image can provide spatial information between 2D and 3D images. The length of the scale bar in the image is 20 μm.