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. 2016 Aug 18;21(8):1083. doi: 10.3390/molecules21081083

Figure 4.

Figure 4

Effects of RAME on IL-1β, IL-10, and IL-6 mRNA expression levels in LPS-treated RAW 264.7 cells. The expression level of pro-inflammatory cytokine genes were measured by real-time PCR. RAW 264.7 cells were treated RAME (12.5, 25, and 50 µM) with LPS (1 µg/mL) and incubated for an additional 18 h. mRNA expression level of genes was normalized with β-actin. (a) IL-1β mRNA expression level; (b) IL-6 mRNA expression level; (c) IL-10 RNA expression level. The results are presented as the means ± SDs of three replicates of one representative experiment. # p < 0.05 vs. control group , * p < 0.05 vs. LPS-treated group.