Figure 3.

Effect of coffee silverskin extract (CSE), chlorogenic acid (CGA), caffeine (CAF) and vitamin C (Vit C) against oxidative damage induced by t-BOOH 0.5 mM. Cells were treated with 0.01–1 mg/mL CSE, 6.88 μg/mL of CGA, 19.86 μg/mL of CAF and 0.1 μg/mL of Vit C for 24 h and further exposed to 0.5 mM t-BOOH for 6 h (A) or 24 h (B). Triton X-100 (10%) was used as the death control. Then, cell viability was measured using the MTT assay. Data represent means ± SD of 18 samples per condition. Different letters denote statistically-significant differences between all treatments (p < 0.05).