Figure 3.

Effects of atractylodin on the phosphorylation of MAPKs in stimulated HMC-1. HMC-1 was treated with the indicated concentrations of atractylodin for 0.5 h prior to being incubated with PMA (50 nM) plus A23187 (1 μM) for 0.5 h. Whole cell lysates were then analyzed by Western blot. Equal amounts of protein (20 μg) were then separated by SDS-polyacrylamide gel electrophoresis and immunoblotted with antibodies.